|
KMID : 0391319920020020169
|
|
Korean Journal of Biological Response Modifiers
1992 Volume.2 No. 2 p.169 ~ p.182
|
|
|
A Simplified Experimental Model for the Induction of Anaphylactic Shock and Cutaneous Reaction Using Compound 48/80
|
|
전병득
강경진/채옥희/송창호/이무삼/신세원
|
|
|
Abstract
|
|
|
Although active systemic anaphylaxis and passive cutaneous anaphylaxis have been empolyed to study anaphylactic hypersensitivity, it is difficult and time-consuming to induce these reactions in experimental animals. The purpose of this study was
to
extablish a simple method to induce anaphylactic hypersensitivity such as anaphylactic shock (AS) and cutaneous reaction (CR) using compound 48/80 To induce AS, various doses of compound 48/80 (5, 7, 10, 15㎍/gm BW.)were injected
intraperitoneally
into
BALB/c mice. Mortility rate, mean death time and mesenteric mast cell degranulation rate were evaluated over a 72 hour period. To induce CR, various doses of compound 48/80 were injected i d into the flank of Sprague-Dawley rats, and the blue
cutaneous
staining induced by Evans' blue injection at the compound 48/80 injection site was observed. As a parameter of these reactions, serum histamine levels, and the levels of histamine, intracellular calcium, and cAMP of rat peritoneal mast cells
(RPMC)
were
measured.
Results were 1) the minimal concentration of compound 48/80 to induce AS in BALB/c mice was 10 ㎍/gm B. W, 2) mean death time resulting from compound 48/80 (10㎍/gm B. W,)-induced AS in BALB/c mice was 21.7 minutes, 3) a dose dependent
relationship
exists between serum histamine levels and concentration of compound 48/80 used to induce AS, 4) the minimal comcentraion of compound 48/80 to induce positive CR in Sprague-Dawley rats was 1.0 ㎍/ml and 5) siginificant increase of mast cell
degranulation
rate in the mesenteries of BALB/c mice and of histamine release from RPMC was induced by compound 48/80 to induce the significant increase of histamine release, intracellular calcium level, and the significant decrease of intracellular cAMP level
of
RPMC was 1.
These data indicate that compound has an ability to induce an anaphylactic shock in very short time and this reaction is due to the vasoactive amine release from RPMC. Therefore, this experimental model could be recommended for the tests o
various
drugs
and ultraviolet radiation-induced cytokines associated with photobiology, allergy and toxicology studies.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|